We currently work at the characterisation of the photosynthetic apparatus of a wide variety of microalgae (e.g., Nannochloropsis gaditana, Chlamydomonas).
Key advances have been reached for Nannochloropsis gaditana, a species belonging to Eustigmatophyceae, a class of eukaryotic algae derived from a secondary endosymbiotic event. To better understand Eustigmatophyceae light exploitation efficiency, PAR-Lab research is developing tools to study their photosynthetic apparatus organization.
We optimized the protocols to purify thylakoid membranes and isolate the different pigment-binding complexes upon mild solubilization and comparing this organization with those known from reference photosynthetic organisms.
The photosynthetic apparatus regulation following different environmental conditions is investigated using instruments such as the PAM (Pulse-amplitude modulation) fluorimeter,the Joliot-type spectrophotometer and the application of HPLC analysis.